P4762
 |  |  |
 | Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. |
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P4762
Sigma
Papain from papaya latex
lyophilized powder, ≥10 units/mg protein (E1%/280 )
| Synonym: | Papainase |
| CAS Number: | 9001-73-4 |
| Enzyme Commission (EC) Number: | 3.4.22.2 ( BRENDA | IUBMB ) |
| EC Number: | 232-627-2 |
| MDL number: | MFCD00131791 |
Description
| Application | Used to produce Fab fragments of antibodies.1 Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.2,3 |
| Biochem/physiol Actions | A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. pH optimum 6.0-7.0 Also hydrolyzes esters and amides. |
| Other Notes | View more information on papain at www.sigma-aldrich.com/enzymeexplorer. |
| Preparation Note | 2× Crystallized |
| Physical form | Lyophilized powder containing sodium chloride and sodium acetate |
Properties
| form | lyophilized powder |
| mol wt | mol wt 21 kDa |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| WGK Germany | 1 |
| RTECS | RU4950000 |
Related Products
| Substrate | P3169, pGlu-Phe-Leu p-nitroanilide |
| Inhibitor | A6191, Antipain dihydrochloride from microbial source |
| M6159, α2-Macroglobulin from human plasma | |
| C7255, Cystamine dihydrochloride | |
| C8917, Cystatin from chicken egg white | |
| L2023, Leupeptin trifluoroacetate salt | |
| E3132, E-64 | |
| G5386, Gly-Gly-Tyr-Arg | |
| C7268, Chymostatin | |
| E3520, Ebselen | |
| D7910, 3,4-Dichloroisocoumarin |
References
| Cited Reference | 1. Y. Ozari, J. Jagur-Grodzinski J. Chem. Soc., Chem. Commun., 295, (1974) |
| 2. M.A. Andrews Carbohydr. Res. 194, 1, (1989) Abstract | |
| 3. H. Cai et al. Anal. Chem. 70, 580, (1998) | |
| reference | Fruton, J.S. Adv. Enzymol. 53, 239, (1982) |
| Glazer, A.N., Smith, E.L. The Enzymes, (1971) 3, 501 | |
| Arnon, R. Meth. Enzymol. 19, 226, (1970) | |
| Dreyfus, Cheryl F., Black, Ira B., and, P. Michael Conn, ed. Methods in Neuroscience San Diego , (1990) 2, 10 | |
| , Harlow, E., and Lane, D. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 626-628 | |
| Townes-Anderson, E., et al., Rod Cells Dissociated from Mature Salamander Retina: Ultrastructure and Uptake of Horseradish Peroxidase. J. Cell Biol. 100, 175, (1985) |
